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pbpB [2019-09-16 09:42:20]
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pbpB [2019-09-16 09:42:20]

class B penicillin-binding protein 2B, Pbp2B, required for the assembly of the [SW|cell division ]complex and septal [SW|cell wall synthesis]
locus
BSU_15160
pI
9.23
mw
79.12 kDa
protein length
716 aa Sequence Blast
gene length
2151 bp Sequence Blast
function
septation, [SW|cell division]
product
penicillin-binding protein 2B, member of the [SW|divisome]
essential
yes
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,581,947 → 1,584,097

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • septal cell wall synthesis (this function can be taken over by [protein|5BBA2769A9C0EDB56625F008A55470C2E2885AA4|PBP3]) [pubmed|28792086]
  • assembly of the cell division complex (this is the reason for PbpB essentiality) [pubmed|28792086]

    • Protein family

  • [SW|transpeptidase family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|38FD9805815BBDDD83789F0F402C0FB221B65FF4|SpoVD]

    • [SW|Domains]

  • contains two C-terminal [SW|PASTA domain]s (aa 598-657, 658-714)

    • Structure

  • [PDB|1RP5] (PBP 2x from ''Streptococcus pneumoniae'', 32% identity) [Pubmed|14734544]

    • [SW|Localization]

  • cell membrane [Pubmed|23869552,20870765]
  • localizes to the division site, this depends on [protein|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|FtsW] [Pubmed|27895631,23895585]
  • extracellular (signal peptide) [Pubmed|18957862]
  • localizes to the sporulation septum during sporulation [Pubmed|15758244]
  • during vegetative growth: septal [pubmed|14731276]

    • Expression and Regulation

    Operons

    genes
    [gene|A7131ACFA0CF5C222237C76E502D4AAD1F9BA3E4|mraZ]-[gene|1F894D6895CE13AA006CFE13A702E46C568F6E79|mraW]-[gene|79712F4CF884A660C71B45B3397AEFA5C0AFA683|ftsL]-[gene|EBEFF9E0A524DCDA19382E5401B923B805E4C559|pbpB]
    description
    [Pubmed|8636036,8244929]

    regulation

  • constitutively expressed [Pubmed|15758244]
    • view in new tab

    Biological materials

    GFP fusion

  • 3122 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|EBEFF9E0A524DCDA19382E5401B923B805E4C559|pbpB]::pSG5061 (cat Pxyl-gfpa-[gene|EBEFF9E0A524DCDA19382E5401B923B805E4C559|pbpB]1-825) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC] (due to essentiality of [gene|EBEFF9E0A524DCDA19382E5401B923B805E4C559|pbpB], the strain is xylose-dependent)

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jeff Errington] lab

    • FLAG-tag construct

  • GP2003 ''pbpB-3xFLAG spec'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • labs

  • [SW|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]

    • References

  • 8636036,14731276,8244929,20487272,10652091,19429628,18957862,21219466,20870765,14734544,23895585,24655324,27895631,28792086